Antitumor Activity of Celery Loaded Lipid PLGA-TPGS Nanoparticles in Glioblastoma
Abstract
Glioblastoma (GBM) is a highly aggressive brain tumor characterized by high recurrence and poor prognosis. Natural compounds are good alternatives for treating glioblastoma and are less toxic than synthetic drugs. Apigenin (AGN) is an effective phytochemical with strong antioxidant and anticancer potential. However, due to its high lipophilicity, the therapeutic effect is limited. Therefore, apigenin lipid polymer hybrid nanoparticles (LPHyNPs) were prepared and characterized according to various parameters in this study. The mean particle size (234.100±23.320 nm), PDI (0.330±0.098), zeta potential (-5.403±0.650 mV), EE (54.99±4.13%), etc. Besides, Morphological analysis by SEM showed that the spherical NP had dark nuclei, indicating that the drug was embedded in the nucleus. In vitro release studies showed that continuous release of AGN from LPHyNPs significantly increased the suspension level of AGN (P<0.05). Cell proliferation and apoptosis experiments showed that LPHyNPs could significantly inhibit cell proliferation and promote cell apoptosis. Therefore, the LPHyNPs developed may be an effective therapeutic system for the management of GBM.
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