Preparation and Characterization of Epitope-Based Ratiometric Fluorescent Molecularly Imprinted Polymers
Abstract
In order to solve the problem of difficult detection of neuronal nitric oxide synthase in the screening of neuronal nitric oxide synthase-postsynaptic density95 (nNOS-PSD95) uncoupling agent, this study used 133 amino acids (nNOS1-133) at the nitrogen terminal of nNOS as template molecules, carbon dots and quantum dots as ratio fluorescence recognition elements, SiO2 as matrix for the first time, combined with surface molecular imprinting technology and antigen-determining principle, to prepare ratiometric flurescent molecularly imprinted polymers (RFMIPs). The resulting RFMIPs were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis，exhibiting uniform spherical morphology, which unambiguously confirmed the successful formation of the nanosensor. The result indicates that the synthesized sensors have promising potential for the assay of trace peptides in complex matrices.
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Copyright (c) 2023 Xicheng Yang, Hongjuan Zhang, Hongliang Xin, Yankun Gao
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