Pharmacological Study of Phenolic Components in Parkinson's Disease
Keywords:
Gastrodia elata Bl, Phenols, Caspase-3, BAX, Parkinson's disease, Network pharmacology
Abstract
In this study, cell experiments were conducted to investigate the effects of extracts on cell viability and apoptosis of Parkinson model in vitro, as well as the expression of cysteine protease-3 (Caspase-3) and B lymphocytoma-2-associated X protein (BAX). The results showed that extract of phenols could improve the loss of cell viability and apoptosis induced by MPP+, and inhibit the enhanced expression of Bax and Caspase-3 by MPP+. The potential targets and signaling pathways of phenols in the treatment of Parkinson's disease were predicted by network pharmacology.
References
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[2] Huang JF, Liu Jianhao, Tan Chunfeng, et al. Clinical research on the treatment of Qi and blood deficiency type of Parkinson's disease [J]. Chinese Journal of Basic Medicine of Traditional Chinese Medicine, 2020,26 (08): 1134-1137.
[3] Gao X. Clinical effect of qi nourishing blood and antifibrillation in Qi and blood deficiency Parkinson's disease [D]. Guangxi University of Traditional Chinese Medicine, 2020.
[4] Zhang CC, Shi JS. Research progress on the phenolic components and their central nervous pharmacological effects of Gastrodia elata [J]. Pharmacology and Clinical Practice of Traditional Chinese Medicine, 2019,35 (02): 167-174.
[5] Wang XL, Xing GH, Hong B, et al. Gastrodin prevents motor deficits and oxidative stress in the MPTP mouse model of Parkinson's disease: involvement of ERK1/2-Nrf2 signaling pathway[J]. Life Sci, 2014,114(2): 77-85.
[6] Kumar H, Kim IS, More SV, et al. Gastrodin protects apoptotic dopaminergic neurons in a toxin-induced Parkinson's disease model[J]. Evid Based Complement Alternat Med, 2013,2013: 514095.
[7] Li C, Chen X, Zhang N, et al. Gastrodin inhibits neuroinflammation in rotenone- induced Parkinson's disease model rats[J]. Neural Regen Res, 2012,7(5): 325-331.
[8] Kim IS, Choi DK, Jung HJ. Neuroprotective effects of vanillyl alcohol in Gastrodia elata Blume through suppression of oxidative stress and anti-apoptotic activity in toxin-induced dopaminergic MN9D cells[J]. Molecules, 2011,16(7): 5349-5361.
[9] Yan X, Liu DF, Zhang XY, et al. Vanillin Protects Dopaminergic Neurons against Inflammation-Mediated Cell Death by Inhibiting ERK1/2, P38 and the NF-kappaB Signaling Pathway[J]. Int J Mol Sci, 2017,18(2).
[10] Dhanalakshmi C, Janakiraman U, Manivasagam T, et al. Vanillin Attenuated Behavioural Impairments, Neurochemical Deficts, Oxidative Stress and Apoptosis Against Rotenone Induced Rat Model of Parkinson's Disease[J]. Neurochem Res, 2016,41(8): 1899-1910.
[11] Wang Y, Zhang S, Li F, et al. Therapeutic target database 2020: enriched resource for facilitating research and early development of targeted therapeutics[J]. Nucleic Acids Res, 2020,48(D1): D1031-D1041.
[12] Wishart DS, Feunang YD, Guo AC, et al. DrugBank 5.0: a major update to the DrugBank database for 2018[J]. Nucleic Acids Res, 2018,46(D1): D1074-D1082.
[13] Stelzer G, Rosen N, Plaschkes I, et al. The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses[J]. Curr Protoc Bioinformatics, 2016, 54: 1-30.
[14] Davis AP, Murphy CG, Saraceni-Richards CA, et al. Comparative Toxicogenomics Database: a knowledgebase and discovery tool for chemical-gene- disease networks[J]. Nucleic Acids Res, 2009, 37 (Database issue): D786-D792.
[15] Amberger JS, Hamosh A. Searching Online Mendelian Inheritance in Man (OMIM): A Knowledgebase of Human Genes [J]. Current protocols in bioinformatics, 2017,58: 1-2.
[16] Szklarczyk D, Gable A L, Lyon D, et al. STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets.[J]. Nucleic acids research, 2019,47(D1): D607-D613.
[17] Zhou Y, Zhou B, Pache L, et al. Metascape provides a biologist-oriented resource for the analysis of systems-level datasets.[J]. Nature communications, 2019,10(1): 1523.
[18] Gao M, Zhang W, Liu Q, et al. Pinocembrin prevents glutamate-induced apoptosis in SH-SY5Y neuronal cells via decrease of bax/bcl-2 ratio.[J]. European journal of pharmacology, 2008,591(1-3): 73-79.
[19] Cheng Y, Zhu G, Wang M, et al. Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP(+)-elicited apoptosis.[J]. Neuroscience research, 2009,63(1): 52-58.
[20] Lev N, Melamed E, Offen D. Apoptosis and Parkinson's disease.[J]. Progress in neuro-psychopharmacology & biological psychiatry, 2003,27(2): 245-250.
[21] Cory S, Adams JM. The Bcl2 family: regulators of the cellular life-or-death switch.[J]. Nature reviews. Cancer, 2002,2(9): 647-656.
[22] O'Malley KL, Liu J, Lotharius J, et al. Targeted expression of BCL-2 attenuates MPP+ but not 6-OHDA induced cell death in dopaminergic neurons.[Z]. 2003: 14, 43-51.
[23] Hartmann A, Hunot S, Michel PP, et al. Caspase-3: A vulnerability factor and final effector in apoptotic death of dopaminergic neurons in Parkinson's disease.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2000,97(6): 2875-2880.
[24] Wang Kida, Wang Xingchen, Ji Lin. Experimental validation of the mechanism of action in Parkinson's disease [J]. Chinese Journal of Experimental Formulology, 2020,26 (24): 178-185.
[25] Kumar H, Kim I, More SV, et al. Gastrodin protects apoptotic dopaminergic neurons in a toxin-induced Parkinson's disease model.[J]. Evidence-based complementary and alternative medicine : eCAM, 2013,2013: 514095.
[26] Dhanalakshmi C, Janakiraman U, Manivasagam T, et al. Vanillin Attenuated Behavioural Impairments, Neurochemical Deficts, Oxidative Stress and Apoptosis Against Rotenone Induced Rat Model of Parkinson's Disease.[J]. Neurochemical research, 2016,41(8): 1899-1910.
[27] Kim IS, Choi D, Jung HJ. Neuroprotective effects of vanillyl alcohol in Gastrodia elata Blume through suppression of oxidative stress and anti-apoptotic activity in toxin-induced dopaminergic MN9D cells.[J]. Molecules (Basel, Switzerland), 2011, 16(7): 5349-5361.
[28] Xu HY, Zhang YQ, Liu ZM, et al. ETCM: an encyclopaedia of traditional Chinese medicine[J]. Nucleic Acids Res, 2019,47(D1): D976-D982.
[29] Daina A, Michielin O, Zoete V. SwissADME: A free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules[J]. Sci Rep, 2017,7: 42717.
[30] Daina A, Zoete V. A BOILED-Egg To Predict Gastrointestinal Absorption and Brain Penetration of Small Molecules[J]. ChemMedChem, 2016,11(11): 1117-1121.
Published
2022-11-02
Issue
Section
Original Research Article
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