Simultaneous Detection of Chlamydia Trachomatis, Neisseria Gonorrhoeae, Ureaplasma Urealyticum by Multiplex PCR-Running

  • Qiancheng Jiao Wenzhou Medical University
  • Xiaoling Zhu School of Nursing, Wenzhou Medical University
  • Weiji Zheng School of Laboratory Medicine and Life Sciences, Wenzhou Medical University
  • Yangyang Xie School of Laboratory Medicine and Life Sciences, Wenzhou Medical University
  • Kang Yu The 1rd School of Medicine, Wenzhou Medical University
  • Sheng Ye School of Laboratory Medicine and Life Sciences, Wenzhou Medical University&Zhejiang Yuanchuang Medical Technology Co., Ltd
DOI: https://doi.org/10.18686/aem.v11i2.229
Keywords: Sexually Transmitted Infection, Multiplex PCR, Chlamydia Trachomatis, Ureaplasma Urealyticum, Neisseria Gonorrhoeae

Abstract

Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU) and Neisseria gonorrhoeae (NG) are the most common pathogens of sexually transmitted infections (STIs), frequently founded in urogenital infections, and showed a criminal role in increasing the risk of potential adverse outcomes. In this study a multiplex PCR assay for the simultaneous detection and accurate identification of 3 clinically relevant pathogens of STIs, i.e., CT, NG and UU in a single tube was developed and evaluated. The limits of detection for the multiplex PCR assay were ~10 copies of DNAs per reaction. This assay has comparable clinical sensitivity to the conventional monoplex real-time PCR assay and considerable potential to be routine molecular diagnostic tool for simultaneous identification of STIs at relatively low cost due to multiplexing.

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Published
2022-07-07
Issue
Vol 11 No 2 (2022)
Section
Original Research Article

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